LC-TOF-MS

Liquid Chromatography-Time of Flight-Mass Spectrometry, LC-TOF-MS (Bruker / MictoTOF QIII)

Small Molecules (Metabolites) identification based on accurate mass, fragmentation and true isotopic pattern in MS and MS/MS

Nano Liquid Chromatography-Time of Flight-Mass Spectrometry, NanoLC-TOF-MS (Bruker / MictoTOF QIII)

Large Molecules (Proteins) identification based on accurate mass, fragmentation and true isotopic pattern in MS and MS/MS

The microTOF-Q is a time-of-flight instrument in combination with LC/MS/MS applications. Sample delivery to the source is by Thermo Scientific Dionex UltiMate 3000 UHPLC system/RSLCnano.

This combination of LC and MS allows:

Detection of masses in a complex matrix, identification based on accurate mass and true isotopic pattern in MS and MS/MS
High resolution and mass accuracy MS (selectivity), high resolution chromatography (speed) and high dynamic range and sensitivity

INSTRUMENTS

MicroTOF QIII Bruker Daltonic
Thermo Scientific Dionex UltiMate 3000 UHPLC system
- Thermo Scientific C18 column
Thermo Scientific Dionex Ultimate 3000 RSLCnano
Thermo Scientific C18 column (AcclaimTM RepMap RSLC, 75um x 15cm, 2um, 100A)