Institut Biologi Sistem

 Institute of Systems Biology

Workshop Outline

Gas chromatography coupled to mass spectrometry (GC-MS) is an essential instrument for high-throughput metabolomics studies especially for VOCs profiling. GC-MS utilizes a quadrupole mass spectrometer capable of high-speed scanning, which enables reliable and fast metabolites identification that are biologically relevant. Generally, samples are normally derivatized prior to GC-MS analysis in order to reduce their polarity and facilitate chromatographic separation. The electron ionization (EI) mass spectra obtained are reproducible and suitable for library matching, mass spectral collections being readily available.

Metabolomics is the study of identification and quantification of small molecules present in biological systems. It is a powerful tool comprises of analytical technologies and multivariate analysis for holistically probing large data sets. The massive development in equipment and software for data collection and analyses had led to rapid progress, with NMR and MS being the most important metabolomic platforms with wide applications in chemical, biological, pharmaceutical and medical sciences. High reproducibility is one of the fundamental advantages of NMR spectroscopy. NMR spectrometers are capable of analyzing the identity, structure, concentration, and behavior of molecules without destroying the samples. In addition, NMR metabolomics continues to make significant contributions to the identification of biomarkers to aid in the diagnosis and treatment of various diseases.

Who should attend

Those who are interested in metabolomics and its applications (research scientists, academicians, postdoctoral fellows, and students) are encouraged to attend the workshop.

List of Speakers and Trainers

  • As a pioneer in Metabolomics field in Malaysia, Prof. Dr. Syarul Nataqain Baharum research is focused on the new insight of analytical and biological perspectives of the metabolomics in the field of systems biology, in particularly on the understanding of secondary metabolite production in local herbal plants as well as fluxomics studies. She has expanding her research focus in understanding host-pathogen interaction in aquaculture disease and also green therapeutic mechanism in neurogenerative diseases. In metabolomics research, pipeline of analytical tools such as LCMS, GCMS, CEMS, FTIR and NMR combined with Principal Component Analysis (PCA) is essential for the dynamic high throughput analysis. This frontier analysis which includes mathematical modelling has provided glimpse of cellular biology and fingerprint of functional network in the model organism in her lab. Currently she has published about 100 research articles, 4 books and 6 chapters in books. As project leader, she also received more than RM 4 million research grants in total amount. Under her supervision, 9 PhD students and 17 MSc were graduated. At the moment, she is supervising a total of 21 PhD students and 18 MSc students. She also has filed two patents and granted one Trade Mark (TM) and one Trade Secret.  Her work has been awarded prestigious awards including BioInnovation Awards in 2011 and Selangor My Innovation Award, 2014. She also engaged in collaborative partnership with industries such as Medika Natura Sdn Bhd, AAR Sdn Bhd and FGV Sdn Bhd.

  • If you are interested to collaborate with her, or be part of her research group, please drop her an email to nataqain@ukm.edu.my.

  • ChM. Dr. Kamalrul Azlan Azizan is a research fellow at INBIOSIS, UKM. He completed his PhD in Systems Biology in 2014 at the Universiti Kebangsaan Malaysia (UKM) in 2014. His research interest includes plant and microbial metabolomics as well as the application of chemometrics and multivariate for biomarkers discovery. Currently, he is working on bioprospecting allelochemicals from Wedelia trilobata using chemometrics and predictive models. 

  • ChM. Dr. Kamalrul Azlan Azizan has a strong hands-on experience with GC-MS and LC-MS systems and actively involves in the development and optimization of GC/LC methods, giving guidance through application and troubleshooting assistance. He can be contacted via email; kamalrulazlan@ukm.edu.my 

  • Dr. Ahmed Mediani obtained his PhD in Food Science from Universiti Putra Malaysia in 2016. He then served as a Postdoctoral Fellow at the Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), UiTM. In 2020, he joined INBIOSIS as a research fellow. 

  • His research focuses on the application of metabolomics in natural products and food Science. He has vast experiences in in vivo and in vitro assays on natural products as well as NMR- and LCMS-based metabolomics analyses. As a researcher, He is actively involved in research projects as well as preparing research proposal, particularly in the area of metabolomics, natural product chemistry and food Science. His recent project entitled NMR-based metabolomics approach revealing metabolic signatures and mechanism associated with anti-diabetic effects of Annona muricata

  • Please feel free to visit his research group website or contact Dr. Ahmed Mediani (ahmed@ukm.edu.my) for research and industrial projects collaboration.

Programme

2nd July 2024 (Tuesday) [Introduction]
Venue : Bilik Seminar IVI, Akademi Siber Teknopolis

Time Activity Trainer
8.30 am Registration Pn. Rafidah Ahmad
9.00 am Opening ceremony and introduction Prof. Dr. Syarul Nataqain Baharum
9.10 am Lecture 1 : Fundamental of Metabolomics Prof. Dr. Syarul Nataqain Baharum
10.30 am Tea break -
11.00 am Lecture 2 :  Introduction to Experimental design and quality control in Metabolomics Prof. Dr. Syarul Nataqain Baharum
12.30 pm Lunch break -
2.00 pm Lecture 3: Application of GC-MS, LC-MS and NMR in Metabolomics Prof. Dr. Syarul Nataqain Baharum
4.o0 pm Afternoon break -
4.15 pm Introduction to CODA
GC-MS Service@INBIOSIS
Pn. Rafidah Ahmad
En. Syahmi Afiq Mustaza
5.00 pm END OF DAY 1

3rd July 2024 (Wednesday) [GC-MS]
Venue : Bilik Seminar IVI, Akademi Siber Teknopolis

Time Activity Trainer
9.00 am Introduction to Day 2 -
9.10 am Hands-on 1 GC-MS: Data pre-processing, alignment, filtering and identification using MS-DIAL ChM. Dr. Kamalrul Azlan Azizan
10.30 am Tea break -
11.00 am Hands-on 2 GC-MS: Data pre-processing, alignment, filtering and identification using MS-DIA ChM. Dr. Kamalrul Azlan Azizan
12.30 pm Lunch break -
2.00 pm Hands-on 2 GC-MS: Univariate and Multivariate using open source tool ChM. Dr. Kamalrul Azlan Azizan
4.o0 pm Afternoon break -
4.15 pm Summary, discussions and wrap-up ChM. Dr. Kamalrul Azlan Azizan
5.00 pm END OF DAY 2

4rd July 2024 (Thursday) [NMR]
Venue : Bilik Seminar IVI, Akademi Siber Teknopolis

Time Activity Trainer
9.00 am Introduction to Day 3 -
9.10 am Hands-on 1 NMR: Data Acquisition and Data Processing Dr. Ahmed Mediani
10.30 am Tea break -
10.45 am Hands-on 2 NMR: Data Acquisition and Data Processing Dr. Ahmed Mediani
1.00 pm Lunch break -
2.00 pm Hands-on 3 Pathway analysis for metabolomics Dr. Ahmed Mediani
4.o0 pm Afternoon break -
4.15 pm Summary, discussions and wrap-up Dr. Ahmed Mediani
5.00 pm
  • Presentation of attendance certificate

END OF DAY 3

Register now or contact us for more details