CRISPR-Cas Technology: A Practical Course for Cas9-mediated Microbial Genome Engineering
CRISPR-Cas genome editing tools are revolutionizing molecular biology and biotechnology R&D.
Concept and applications of CRISPR-Cas technology
Learn the fundamentals and applications of CRISPR-Cas technology. We will go through CRISPR-Cas system selection and experimental design training for RNP-based CRISPR-Cas9 genome editing.
CRISPR-Cas9 sgRNA design and synthesis
Learn how to design, evaluate and synthesize CRISPR single guide (sgRNA). We will go through interactive design and analysis of guide sequence and in vitro synthesis of target-specific sgRNA.
Validation of Cas9 RNP gene editing and mutation detection
Learn how to conduct genome editing of model yeast Saccharomyces cerevisiae using Cas9 RNP-based system. We will go through interactive Cas9 cleavage efficiency and mutation detection assays important for validation of Cas9-mediated genome editing.
Who should attend
Postgraduate students (Master and PhD students), Post Doctorate, Academicians, Researchers (Government and non-government institution) and Life Science Service Providers (Company, agency).
List of Speakers and Trainers
Dr. Ahmad Bazli Ramzi received his Bachelor and PhD degrees from University of Malaya (Malaysia) and Korea University (South Korea), respectively, with major in Biotechnology. He assumed post-doctoral fellow position and currently, works as a research fellow at the Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM), Malaysia. He is actively working with national and international institutions for promoting microbial synthetic biology research through various R&D platforms including as plenary and invited speaker at 12th Malaysia International Genetics Congress 2017 (MiGC12), Symposium on Promoting Institutional Oversight to Address Biosecurity Threats from Emerging Technologies 2019 and AFOB-MC International Symposium (AFOBMCIS) 2021.
He is the principal investigator for several research projects awarded by UKM and national agencies including Yayasan Penyelidikan Antartika Sultan Mizan (YPASM) Fellowship and Ministry of Education (MOE) FRGS Scheme. Dr. Bazli has established international partnership with United Kingdom (UK) synthetic biology centres at University of Manchester (SYNBIOCHEM, UoM) and University of Nottingham (SBRC Nottingham, UoN) where his collaborative projects have received funding from UoN International Collaboration Fund and Global Doctoral Research Network (GOLDEN) Global Challenges Research Fund (GCRF) - PhD Studentship 2020 programmes.
His research group pursued towards integrating the multidisciplinary fields of Biological Engineering, Systems Biology and Synthetic Biology as biotechnological tools for translational research in Malaysia. With the aim of promoting industrial and CRISPR biotechnology, Dr. Bazli group specialize in chassis optimization, bioproduction (Specialty and fine chemicals), biotherapeutics (Engineered live biotherapeutics) and CRISPR technology (CRISPR-Cas9 and Cas13) using in-house Bioengineering and iCRISPR modules. Using omics-guided approaches, his group seeks to develop innovative solutions for addressing environmental and industrial problems including plastic waste pollution (Plastic bioconversion and biomonomer production) and oil palm basal stem rot disease (Ganoderma functional and biopesticide studies). Dr. Ahmad Bazli can be contacted via email; firstname.lastname@example.org.
Dr. Hamidun Bunawan obtained his PhD and received intensive synthetic biology training at Bristol University, United Kingdom. His research activities have been centred around the investigating range of interconnecting themes that include bioengineering, biotechnology, systems and synthetic biology.
His signature project comprises constructing the synthetic virus of Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) as a powerful platform to delve deeper into the function of viruses. His research interest also extends to the microbial host’s metabolic engineering, mainly using yeast to produce plant anthocyanin and genetic manipulation of antibiotics produced by Lactobacillus and Trichoderma.
Please feel free to contact Dr. Hamidun Bunawan (email@example.com) for research and industrial projects collaboration.
18th May 2022 (Wednesday)
|0900||Welcome and Introduction||Bioinfo. Lab / Seminar Hall|
|0910||CRISPR technology talk session
||Bioinfo. Lab / Seminar Hall|
|1100||Demonstration session 1: Designing guide RNA using in silico tools||Bioinfo. Lab / Seminar Hall|
|1400||Hands-on session 1: Synthesis and purification of CRISPR sgRNA||Genomics Lab|
|1700||End of session / Tea break||Foyer|
|1730||End of Day 1
19th May 2022 (Thursday)
|0900||Demonstration session 2: Design for CRISPR-Cas-mediated gene knock-in||Bioinfo. Lab / Seminar Hall|
|1100||Hands-on session 2: Assembly and in vitro cleavage assay of Cas9/sgRNA RNP||Genomics Lab|
|1400||Hands-on session 3 : Determination of gene mutation via T7E1 assay||Genomics Lab|
|1700||End of session / Tea break||Foyer|
|1730||End of Day 2