Institute of Systems Biology

 Institute of Systems Biology

Workshop Outline

This training course is held to give participants exposure to basic knowledge in plant tissue culture, including the latest theory and applications. Tissue culture is an important technique to develop to be used as a support system before further studies involving fields such as molecular biology, transcriptomics, proteomics, metabolomics, cell engineering, and systems biology. Tissue culture techniques can also be applied to the development of agricultural aspects, for example for the large-scale production of clones of interest. The basis of tissue culture can also be used for the preservation and conservation of endangered species, through cryopreservation methods.

Who should attend

Postgraduate students (Master and PhD students), Post Doctorate, Academicians and Researchers (Government and non-government institution)

List of Speakers and Trainers

  • Dr. Emelda Rosseleena Rohani is currently a fellow researcher at the Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM). She obtained her Ph.D. in pharmaceutical sciences from Chiba University, Japan, in 2016. She earned a Bachelor’s and Master's degree in Plant Biotechnology from UKM. Her strength is in plant tissue culture studies, involving direct or indirect organogenesis, somatic embryogenesis, cell suspension culture, as well as histology and anatomy analysis. The plant of interest covers tropical plant species such as mangosteen and Garcinia spp., Nepenthes spp. (the carnivorous plant), Nephelium spp. and Citrus spp.

  • Her current research focuses on the papaverine or alkaloids content in Sauropus androgynus or locally known as pucuk manis. She is also currently working on understanding the behaviour of recalcitrant seed and using mangosteen seed as a model study. The findings would perhaps help conserve and store the seeds, thus preserving the country's biodiversity. If you are interested in collaborating with her, being part of her research group, or have any inquiries regarding the Research Plot, please email her at emelda@ukm.edu.my.

  • Prof. Emeritus Dr. Normah's research group focuses on understanding recalcitrant seed behaviour by using systems biology approach. They are actively involved in the development of techniques for cryopreservation of species with recalcitrant seeds. They successfully developed several techniques for the long-term conservation and micropropagation of economically (and traditionally) important species such as the Garcinia, Nephelium and Citrus species. They have expanded research to include the broad areas of genetic diversity, developmental biology, molecular biology and metabolomics. These are the newest cutting edge areas for cryopreservation and genetic resources preservation.

Programme

18 Oktober 2022 (Tuesday), Webex

Time Activity
  8.30 am Registration
  9.00 am Introduction
  9.10 am Lecture 1: Principles and Application of Plant Tissue Culture -Prof. Emeritus Dr. Normah Mohd Noor
10.30 am Morning break
11.00 am Lecture 2: Aseptic Techniques and Surface Sterilization 
12.30 pm Lunch
2.00 pm Lecture 3: Media Preparation
3.00 pm Lecture 4: Micropropagation: Shoot induction, rooting and Explant Sub-culture
4.00 pm Tea break
5.00 pm Participant flash talks

Summary, discussions, and wrap-up

4.30 pm End of Day 1

 

19 Oktober 2022 (Wednesday), Webex

Time Activity
9.00 am Introduction of Day 2
9.10 am Lecture 5:  Experimental Design and Observation
10.30 am Morning break
11.00 am Lecture 6: Callus Culture and Somatic Embryogenesis
12.30 pm Lunch
2.00 pm Lecture 7: Cryopreservation
3.00 pm Tea break
4.00 pm Participant flash talks

Summary, discussions, and wrap-up

4.30 pm End of Day 2

 

20 Oktober 2022 (Thursday), Pusat Bioteknologi Tumbuhan, INBIOSIS UKM

Time Activity
9.00 am Introduction of Day 3
9.10 am Hands-on 1: Media Preparation
10.30 am Morning break
11.00 am Hands-on 2: Sample Sterilization
12.30 pm Lunch
2.00 pm Hands-on 3: Seed Culture
3.30 pm  Tea break
4.00 pm Lab Tour, Summary, Discussions, and Conclusion
4.30 pm End of workshop

Register now or contact us for more details